NMDA induces prostaglandin E2 production in mixed and neuronal-enriched cultures. Media from mixed (A) and neuronal-enriched (B) cultures were analyzed by ELISA for the presence of PGE2 from cultures either treated with NMDA or vehicle (control). The amount of PGE2 detected (pg/ml) is plotted for the average (+SEM) from 4 independent experiments. Note that the scales of PGE2 differ between preparations. (**indicates P = 0.01 in comparison to vehicle treatment by ANOVA, Tukey-Kramer multiple comparison test).