Lack of neuroprotection by EP1 receptor antagonists or NS398 in mixed cultures. Ono 8711, SC51089 and NS398 did not confer neuroprotection in mixed cultures. Mixed cortical cultures were treated with the concentrations of Ono 8711 indicated, 10 μM SC51089 or 30 μM NS398 followed by treatment with NMDA (average [NMDA] = 20 μM). These cultures were treated in parallel with the neuronal-enriched cultures shown in Figure 2. Neuroprotection was not observed with any concentration of Ono 8711, SC51089, or NS398. The results shown were the average (+SEM) from four independent experiments.