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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Ciliary neurotrophic factor (CNTF) plus soluble CNTF receptor α increases cyclooxygenase-2 expression, PGE2release and interferon-γ-induced CD40 in murine microglia

Figure 5

CNTF in combination with sCNTFRα potentiates the effect of IFNγ on CD40, but not MHC class II, expression in murine microglia. Microglia were treated with IFNγ (10 ng/mL) (IFN), IFNγ (10 ng/mL) plus CNTF (10 ng/mL) (IFN+CN), or IFNγ (10 ng/mL) plus the combination of CNTF (10 ng/mL) and sCNTFRα (200 ng/mL) (IFN+CN+R), or left untreated (UT) for 24 hours. 10,000 cells were analyzed by flow cytometry for CD40 and MHC class II expression. Panel A shows representative histograms for CD40 (APC) while Panel B shows representative histograms for MHC class II (FITC). C, Mean fluorescence of CD40 and inset shows averaged values from three independent experiments as normalized to IFNγ alone. D, Mean fluorescence of MHC class II; E, Percentage of CD40 positive cells; F, Percentage of MHC class II positive cells. Values represent the means ± S.E.M. from triplicates in one experiment. Different superscript letters indicate significant differences at the p < 0.05 levels as analyzed by one-way ANOVA followed by Tukey's post-hoc test. Data are representative of 3 independent experiments.

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