Figure 6

CNTF in the presence of sCNTFRα potentiates the effect of IFNγ on CD40, but not MHC class II, expression in dendritic-like microglia. After continuous growth in GMCSF (10 ng/mL) for 7 days, the dendritic-like microglia were treated with CNTF (10 ng/mL) (CN), a combination of CNTF (10 ng/mL) and sCNTFRα (200 ng/mL) (CN+R), IFNγ (10 ng/mL) (IFN), IFNγ (10 ng/mL) plus CNTF (10 ng/mL) (IFN+CN) or IFNγ (10 ng/mL) plus the combination of CNTF (10 ng/mL) and sCNTFRα (200 ng/mL) (IFN+CN+R) or left untreated (UT) for 24 hours. 10,000 cells were analyzed by flow cytometry for CD40 and MHC class II expression. Panel A and C show representative histograms for CD40 expression while Panel B and D show representative histograms for MHC class II expression. E, Mean fluorescence of CD40; inset shows data from three independent experiments, IFNγ, IFNγ plus the combination of CNTF and sCNTFRα. F, Mean fluorescence of MHC class II; G, Percentage of CD40 positive cells and inset shows data from three independent experiments. H, Percentage of MHC class II positive cells. Values represent the means ± S.E.M. from triplicates in one experiment. Different superscript letters indicate significant differences at the p < 0.05 levels as analyzed by one-way ANOVA followed by Tukey's post-hoc test. Data are representative of 3 independent experiments.