Figure 1

CD133, COX-2 and MT1-MMP expression are increased in U87 neurospheres glioma cultures. (A) U87 glioblastoma-derived cell lines were cultured as monolayers or non-adherent neurospheres as described in the Methods section and representative phase contrast photographs were taken. (B) Cell lysates were isolated from U87 glioblastoma-derived cells and SDS-PAGE performed (20 μg protein/well), followed by Western blotting and CD133, COX-2, MT1-MMP or GAPDH immunodetection. Gelatin zymography was also used to monitor the extent of latent proMMP-2 and active MMP-2 expression from the conditioned media of the serum-starved cells.