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Figure 3 | Journal of Neuroinflammation

Figure 3

From: A MT1-MMP/NF-κB signaling axis as a checkpoint controller of COX-2 expression in CD133(+) U87 glioblastoma cells

Figure 3

CD133-sorted U87 glioma cells grow as neurospheres and express high levels of COX-2. (A) U87 CD133(+) cells were isolated from the parental U87 cells as described in the Methods section using MACS technology. Evaluation of CD133 cell surface expression was then performed by flow cytometry on parental U87 and CD133(+) sorted cells. (B) U87 CD133(+) cells were put back into culture and show a typical neurosphere phenotype, unlike their parental counterpart. (C) Total RNA was extracted from parental U87 and CD133(+) U87 cells and gene expression levels were assessed by qRT-PCR for CD133 (white bars), β-actin (black bars), MT1-MMP (lined bars) and COX-2 (grey bars).

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