Figure 5

COX-2 induction by MT1-MMP occurs through NF-κB-mediated mechanisms. Wild-type (Wt), p65^-/-, and p50^-/- mouse embryonic fibroblasts (MEF) were Mock-transfected or transfected with a cDNA plasmid encoding MT1-MMP. Gelatin zymography was used to monitor the extent of latent proMMP-2 and active MMP-2 expression from the conditioned media of the serum-starved cells (upper panel). Cell lysates were isolated and SDS-PAGE performed (20 μg protein/well), followed by Western blotting and COX-2 immunodetection.