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Table 1 RT-PCR primer sequences

From: Regulation of CCL2 and CCL3 expression in human brain endothelial cells by cytokines and lipopolysaccharide

GENE

PRIMER SEQUENCE

CCL2

*F: atg aaa gtc tct gcc gcc ctt ctg t

†R: agt ctt cgg agt ttg ggt ttg ctt g

CCL3

F: atg cag gtc tcc act gct gcc ctt

R: gca ctc agc tcc agg tcg ctg aca t

GAPDH

F: cca tgt tcg tca tgg gtg tga acc a

R: gcc agt aga ggc agg gat gat gtt c

  1. PCR primer sequences for CCL2 and CCL3 amplify fragments that are 298 and 274 base pairs in length, respectively. *F-forward/sense primer. †R-reverse/anti-sense primer.