Comparison of infarct volume and brain swelling of CD59a-deficient and wild-type-mice (C57BL/6) after 30 min MCAO. Infarct volumes (A, C, E), as well as volume of brain swelling (B, D, F) given as box-and-whisker-plots are visualized. Statistical analysis was performed with the Mann-Whitney-U-test (*: p ≤ 0.05). In contrast to 1 h MCAO-model (figure 1), after 30 min MCAO and 72 h of reperfusion there was a significant difference of infarct volumes and brain swelling between wild-type and CD59a-deficient mice in both, mixed gender and male mice: infarct volumes were larger and brain swelling increased in male CD59a knockout mice (nCD59a-/- m = 22; nWT m = 18), as well as in knockout mice of mixed gender (n = 25CD59a-/- mix; n = 24WT mix), when compared to age- and gender-matched wild-type control mice. No significant differences were seen for female mice. In all box plots, the top of the box represents the 75th percentile, the bottom of the box represents the 25th percentile, and the line in the middle represents the 50th percentile. The whiskers (the lines that extend out the top and bottom of the box) represent the highest and lowest values that are not outliers or extreme values.