Figure 1
From: Reduction of β-amyloid pathology by celastrol in a transgenic mouse model of Alzheimer's disease
A) Chemical structure of celastrol, an active ingredient of the "Thunder of God" medication which has been used as an anti-inflammatory remedy for centuries in China. B) Dose dependent effect of celastrol on NFκB activation induced by TNFα in HEK293 cells stably expressing an NFκB luciferase reporter construct. Cells were co-treated with 20 pg/ml of TNFα and with a dose range of celastrol for 3 hours before measuring NFκB luciferase activity. ANOVA reveals a statistically significant main effect of celastrol dose on NFκB activation (P < 0.002). Post-hoc analysis show significant differences between NFκB activity in TNFα treated cells and cells co-treated with TNFα and celastrol for celastrol doses greater or equal to 10 nM (P < 0.005). C) The histogram represents the amounts of LDH released detected in the culture medium of HEK293 cells treated with a dose range of celastrol and with the MPER lysis buffer as a positive control (lysis buffer) to induce complete cellular lysis. ANOVA reveals no significant main effect of celastrol dose (P = 0.420) and a significant effect of the lysis buffer (P < 0.001) on LDH released.