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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Norepinephrine enhances the LPS-induced expression of COX-2 and secretion of PGE2 in primary rat microglia

Figure 1

Different concentrations of norepinephrine enhance the synthesis of COX-2 protein (A, B) and mRNA (C) in LPS-stimulated primary neonatal microglial cells. Microglial cells were stimulated with different concentrations of norepinephrine (0.001 μM to 10 μM) and LPS (10 ng/ml) for 4 hours. (A) A representative western blot against β-actin (42 kDa) and COX-2 (70 kDa) is shown here. (B) Quantitative densitometric analysis of COX-2 protein expression normalized to β-actin control (n = 3). *P < 0.05, **P < 0.001 with respect to LPS control. (C, D) Semi-quantitative PCR analysis of the effect of different concentrations of norepinephrine alone (C) and of norepinephrine plus LPS on COX-2 and COX-1 mRNA expression (D). [NE = norepinephrine].

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