Effects of HgCl
on (A) β-hexosaminidase and (B) VEGF release from LAD2 mast cells. LAD2 mast cells were incubated with HgCl2 and/or SP as indicated for 10 min for β-hexosaminidase and 24 hours for VEGF release at 37°C. The cells were then centrifuged and the supernatant fluid was collected. VEGF release was assayed by ELISA, while β-hexosaminidase release was assayed spectrophotometrically (n = 5, *p < 0.05).