Figure 11

No evidence for antibodies to Homer3 (A) or the metabotropic glutamate receptor 1 alpha (mGluR1α) (B) in the patient's CSF as demonstrated by double staining with commercial antibodies to these antigens. While the patient's CSF preferentially stained Purkinje cell dendrites on mouse (Panel A and B) and monkey (Panel A, inset) tissue, Homer3 and mGluR1a immunoreactivity was widely restricted to dendritic spines. IgG antibodies to Homer3 and mGluR1α are depicted in red (AF568); the patient's IgG is labeled in green; yellow color indicates overlay of the two antibodies. Nuclei are shown in blue (DAPI).