Figure 12
No evidence for antibodies to the glutamate receptor delta 2 (GluRδ2) (A) or the protein kinase C type gamma (PKCγ) (B) in the patient's CSF as demonstrated by double staining with commercial antibodies to these antigens. Note that the anti-GluRδ2 antibody as well as the anti-PKCγ antibody stained interneurons on mouse (A and B; arrows) and primate (B, left upper inset; arrows) cerebellar tissue, which were spared by the patient's antibody (cf. Figure 5). The anti-PKCγ antibody also bound to dendritic spines on monkey tissue that were not stained by the patient's antibody (B, right upper inset). In contrast to the patient's CSF, anti-PKCγ stained capillaries in primate brain (asterisk). Moreover, the cytoplasmic staining found with the anti-PKCγ antibody exceeded that caused by the patient's antibody and appeared to include the nuclear membrane (B, lower inset; left and right images represent different exposure times). IgG antibodies to GluRδ2 and PKCγ are depicted in red (AF568); the patient's IgG is labeled in green; yellow color indicates overlay of the two antibodies. Nuclei are shown in blue (DAPI).