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Figure 9 | Journal of Neuroinflammation

Figure 9

From: A new Purkinje cell antibody (anti-Ca) associated with subacute cerebellar ataxia: immunological characterization

Figure 9

Double staining of cultured Purkinje cells (PC) with the patient's serum revealed binding of IgG to PC somata and to membranes of PC dendrites and axons. Calbindin is depicted in green (A; AF488); the patient's antibody in red (B; AF555); yellow color indicates overlay of the two antibodies (C). Binding was only observed after fixation with 4% paraformaldehyde and 0.05% triton-X (A-C), but not on living cells (D-E), suggesting an intracellular localization of the target antigen. Panel D shows fluorescence of a GFP-transfected living (i.e. unfixed) PC; Panel E demonstrates lack of binding of the patient' serum to the same cell.

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