Figure 5
From: Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury
Treatment with DAF suppresses NaCN induced expression of C3 protein in neurons. Primary neurons in glucose-free medium were exposed to 1.5 mM of NaCN for 1 hour then the cells were bathed in normal medium with or without 200 ng/ml of DAF. Expression and distribution of C3 in the neurons was assessed by immunofluorescent staining using anti-C3 (green), anti-NF200 (red), and DAPI (blue) antibodies (n = 3). Original magnification: ×400. Scale bar = 50 μm.