DAF suppresses the expression of activated caspase in neurons exposed to chemical hypoxia. Primary rat cortical neurons were exposed to glucose free medium containing 1.5 mM NaCN for 1 hr then allowed to recover overnight in normal medium with or without DAF. (a) Cell lysates were analyzed by immunoblot using anti-active caspase-3, anti-caspase-9, and anti-β-actin antibodies. (b) Data was quantified by densitometry (n = 10). (c and d) Cells were marked with anti-active caspase-3 (red), anti-MAC (green) antibodies, and DAPI (blue) (n = 3). Scale bar = 50 μm. (one-way ANOVA followed by Newman-Keuls test. *p < 0.05).