NaCN does not affect expression of endogenous rat DAF (rDAF) and recombinant human DAF is able to anchor to neuronal cells. Primary rat neurons were cultured under chemical hypoxic conditions for 1 hour then recovered in normal medium with or without recombinant human DAF. Expression and distribution of rDAF and recombinant human DAF (hDAF) in neurons were shown by immunostaining with anti-rat DAF(red), anti-human DAF(green) antibodies, and DAPI (blue) (n = 2). Original magnification: ×400. Scale bar = 50 μm.