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Figure 2 | Journal of Neuroinflammation

Figure 2

From: TGF-β1 blockade of microglial chemotaxis toward Aβ aggregates involves SMAD signaling and down-regulation of CCL5

Figure 2

TGF-β1 suppressed Aβ-induced microglial chemotaxis in the transwell assay. BV-2 cells were seeded in the inserts of transwells with or without 10 ng/ml TGF-β1. The inserts were transferred to transwells containing 10 μM Aβ25-35 and incubated for 2 h. Images of control, TGF-β1-, and Aβ-treated BV-2 cells, with and without TGF-β1 treatment, were taken from four random fields at 4× magnification (A). The cell number in the lower chamber of the insert was quantified (B). Primary microglia were seeded in inserts of transwells with or without TGF-β1 at different concentrations and incubated for 30 minutes. The cells in the lower chamber of the insert were quantified (C). Bars represent the mean ± SEM. * = p < 0.05.

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