Figure 2

Luteolin triggers global transcriptomic changes in non-activated and LPS-activated BV-2 microglia. (A) Comparative Gene Expression Dynamics Inspector (GEDI) analysis of DNA-microarray datasets from control BV-2 cells or cells treated with 50 μM luteolin, 50 ng/ml LPS, or 50 μM luteolin + 50 ng/ml LPS. The white circle denotes the most prominent expression changes in several corresponding gene clusters. (B) Genomatix ChipInspector single probe analysis of differentially expressed transcripts in luteolin versus control-treated cells (top) or luteolin + LPS treated cells versus LPS stimulated cells. Triplicate Affymetrix Mouse 430 2.0 GeneChips were analyzed with a false-discovery-rate (FDR) <0.1%, 3 significant probes and log2 ratios >1. Differentially expressed transcrips from both comparisons were subjected to pathway analysis and clusters with ≥ 3 genes were defined as an independent group. Up-regulated genes are shown on the left side in red and down-regulated genes are displayed on the right side in blue. The total numbers of significantly up- or down-regulated genes from both comparions are indicated in circles placed in the centers of individual pie charts. All differentially expressed genes are listed in Tables 2 and 3, respectively.