DMF inhibition of ERK phosphorylation in LPS-activated microglia. Results are compared to microglial cultures stimulated solely by LPS. Results are detected by western blot after 5 minutes of treatment. Activated ERK (phospho-ERK) species were detected by immunoblot analysis with antibodies specific for the phosphorylated forms of the kinase (A). The amount of protein loaded in each lane was confirmed by measurement of the amount of ERK using antibody against the unphosphorylated form of ERK. (B) Blots were subjected to densitometry and normalized to the signal of the respective pan-MAPK antibody. Results are representative of 3 independent experiments (*** = p < 0.001).