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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Chronic upregulation of activated microglia immunoreactive for galectin-3/Mac-2 and nerve growth factor following diffuse axonal injury

Figure 1

Representative images of sections from control experiments of immunofluorescence labelling. Panels A and B show immunoreactivity within the white matter for NGF (A) and galectin-3/Mac-2 (B). In panels C and D, sections were exposed to either goat anti-galectin-3/Mac-2 antibody (C) or rabbit anti-NGF antibody (D), but were then subsequently exposed to the inappropriate secondary antibody (FITC chicken anti-rabbit for galectin-3/Mac-2 (C) or Alexa Fluor 594 donkey anti-goat for NGF (D)). Panels E and F: Primary antibody was omitted and sections were exposed to either FITC chicken anti-rabbit (E) or Alexa Fluor 594 donkey anti-goat (F). These experiments verified that secondary antibodies did not bind to an inappropriate antigen in tissue sections. All tissues were from post-TBI (day 8) animals. Bar = 20 μm.

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Journal of Neuroinflammation

ISSN: 1742-2094