Galectin-3/Mac-2 immunoreactive microglia are also immunoreactive for NGF. Representative confocal photomicrographs illustrating co-localization of NGF-ir (A, arrows) and galectin-3/Mac-2-ir (B, arrows) microglia in an animal 7 days post-injury. Image overlay of NGF and galectin-3/Mac-2 labelling shows that the majority of galectin-3/Mac-2+ microglia are also immunoreactive for NGF. Semi-quantitative evaluation of percentage of double-labeled cells in all experimental groups confirms the qualitative observation; 93-99% of all galectin-3/Mac-2+ microglia are immunoreactive for NGF (D). Bar = 20 μm in A-C. Orthogonal reconstructions (E-G) from confocal z-series in x-z (top) and y-z (right) planes confirm that NGF-ir and galectin-3/Mac-2-ir are present within the same cell (arrows).