Figure 2
P2X 7 r activation triggered astrocyte-mediated neurotoxicity by inducing oxidative stress. (A) Motor neuron survival in co-culture with astrocytes pre-treated with ATP (100 μM, 5 days) or BzATP (10 μM, 48 hours) and the P2X7r inhibitor BBG (1 μM). (B) Motor neuron survival in co-culture with astrocytes pre-treated with NAME (1 mM), MnTBAP (0.1 mM) or urate (0.2 mM) and BzATP before co-culture. Data are expressed as the percentage of control, mean ± SEM from at least three independent experiments. *p < 0.05, significantly different from untreated control.