HSV-1-infected TLR2-/- microglial cells have attenuated intracellular ROS production compared to wild-type microglial cells. Microglial cell cultures from wild type and TLR2-/- mice were infected with HSV-1 (MOI = 2) and measured, at various time points, for ROS production. DCFH-DA (20 μM) was added 30 min before each time point and measured with a fluorescence plate reader. Data are normalized to ROS production in the uninfected control microglia and presented as the % of control ± SD and are representative of three separate experiments. * = p < 0.05.