Figure 2

Croton oil treatment of α7KO mice selectively enhances neutrophil infiltration into ears 6 hours post-croton oil application. A) Flow cytometry of infiltrating cells from the α7WT (upper) or α7KO (lower) mice reveals a substantial difference in the infiltration by Ly6G⁺ neutrophils 6 hours following croton oil treatment. Similar results were also obtained for C3H mice (data not shown). Histogram height is displayed as Percentage of Maximum (% Max), meaning that peak heights within each graph are normalized to a percentage of the peak representing the highest event number on the X axis. The percent of Ly6G positive cells is shown above the peak response. At least 3 independent experiments, each containing 5 mice per group, are shown and isotype controls for all antibodies showed a staining pattern identical to the unstained controls (shaded histogram). B) Infiltrating cells stained with other immune cell markers for α7WT and α7KO genotypes. C) Bone marrow granulocytes of α7WT and α7 KO mice double labeled for Ly6G and the more immature marker, Ly6C (granulocytes and neutrophils). Unlike infiltrating cells to the skin, these cells do not differ in resident bone marrow populations. D) Similar to Panel A, either α5KO mice or β4KO mice with corresponding WT controls were examined for Ly6G recruitment to the skin. The grey shaded histogram represents control unstained cells. E) Cells from ears of α7WT and α7KO mice were harvested 24 hours post application of croton oil. Results reflect similar numbers of Ly6G positive cells at this later time.