Figure 4

Ly6G⁺ infiltrating cells co-express CCR10. A) Ly6G⁺/CCR10⁺ infiltrating cells 6 hours after croton oil. Ly6G cells from the skin express low levels of CCR10 (CCR10^lo). There are more Ly6G⁺CCR10^lo cells present in the α7KO (32%) versus the WT (18%). The numbers reflect percentage of cells rounded to the whole number. Analysis of bone marrow or spleen reveal no difference in neutrophil numbers relative to genotype as reported [28, 30]. Isotype controls were identical to unstained controls (not shown). B) The percentage of Ly6G⁺CCR10^lo cells elicited by croton oil application to the β4WT or β4KO mice was equivalent. C) Croton oil induced infiltrating cells were also examined for co-expression of CCR10 with other immune cell markers including macrophages (F4/80), B-cells (B220) and T-cells (CD4 or CD8).