Enhanced cytokine production by MPS IIIB lymphocytes. Donor splenocytes isolated from 2-3-month-old MPS IIIB (n = 8) or wt mice (n = 8) were pooled and incubated for 48 hrs in RPMI 1640 growth media in the presence of ConA (1 μg/ml). The media were then assayed for cytokines by Bio-plex assay. The data here were means from two sets of independent experiments (n = 8/group/experiment). Each sample was analyzed in duplicates. +/+: wt; -/-: MPS IIIB. *: P < 0.01; #: P < 0.05.