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Table 3 Effect of adoptive transfer of MPS IIIB lymphocytes on the expression of immune-associated genes in the brain of recipient mice

From: Mucopolysaccharidosis IIIB, a lysosomal storage disease, triggers a pathogenic CNS autoimmune response

Gene symbol Gene name GenBank access # #2-ΔΔCt (fold of increase)
IFN-γ Interferon gamma NM_008337 17.8*
CD68 CD68 antigen NM_009853 5.2*
GFAP Glial fibrillary acidic protein NM_010277 4.1*
IL4 Interleukin 4 NM_021283 2.8*
IL5 Interleukin 5 NM_010558 1.7*
IL6 Interleukin 6 NM_031168 1.3
IL17 Interleukin 17 NM_010552 2.1*
IL2 Interleukin 2 NM_008366 1.9*
TNF-α Tumor necrosis factor alpha NM_013693 1.9*
CD45 Protein tyrosine phosphatase receptor type C NM_011210 1.7*
Ifi30 Interferon gamma inducible protein 30 NM_023065 1.8*
IFN-α Interferon alpha NM_008334 1.6*
CD3e CD3 antigen, epsilon polypeptide NM_007648 1.3*
CD4 CD4 antigen NM_013488 1.4*
CD8a CD8 antigen, alpha chain NM_001081110 1.2
IL10 Interleukin 10 NM_010548 1.0
  1. #Real-time qRT-PCR data were analyzed by means of the comparative threshold (CT) method using mouse GAPDH as internal control and expressed as 2-ΔΔCt. These changes reflect relative increases in gene expression in the brain of mice receiving MPS IIIB lymphocytes (n = 4) as compared to wild-type lymphocytes-treated mice (n = 4). *P < 0.05 (ΔΔCt of MPS IIIB lymphocytes-treated mouse brains vs. wild-type lymphocytes-treated mouse brains).