Figure 7
Changes in SK3 immunoreactivity and microglia/macrophages after an intracerebral hemorrhage (ICH). Confocal images in A-C were labeled with OX-42 antibody (mouse monoclonal; 1:200) and a Cy3-conjugated secondary (donkey anti-mouse, 1:400). A. A ring of activated microglia/macrophages surrounds the hematoma at 7 days after an ICH: c, centre; e, edge; s, surrounding striatum (scale bar, 500 μm). B. In the normal striatum, there is widespread OX-42 labeling of 'resting' microglia (scale bar, 100 μm). The inset shows a typical ramified microglial cell with long processes and a small cell body (scale bar, 20 μm). C. At the edge of the hematoma 7 days after an ICH, microglia/macrophages transition to amoeboid cells with partially retracted processes further from the lesion (and inset at upper right) to round cells near the hematoma (and left inset). Scale bars: 100 μm for the main image and 20 μm for the insets. D. At the edge of the hematoma, SK3 labeling in microglia/macrophages (as in Figure 6) is shown in high-magnification color-separated images (scale bars, 10 μm). The negative control (inset in D') shows lack of staining with Cy3-conjugated 2° antibody alone. The merged image (D'') shows extensive surface SK3 staining in activated microglia/macrophages (examples marked with arrows).