Figure 7
IL-6 is expressed in neurons and astrocytes following GD-induced SE. IL-6 (green) is present in the piriform cortex, hippocampus and thalamus 12 hours after GD-induced SE (A, B & C; left), though absent in vehicle controls (A, B & C; right). IL-6 co-localized with neurons (D, red) and to a lesser extent, astrocytes (E, red). No co-localization was observed in microglia (F, red). DAPI (A-F, blue) labels nuclei. For confocal images, the white lines indicate the vertical (Z) sectioning of the image and are shown as Z1 and Z2 on the edges of the main image. (Scale bar: 250 μm (A-C), 50 μm and 10 μm (D-F) for regular and confocal fluorescent microscopy respectively; n = 3 for each cytokine/cell type combination.)