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Table 1 Antibodies used for immunoblotting (IB) and immunohistochemistry (IHC)

From: Gp91phox (NOX2) in classically activated microglia exacerbates traumatic brain injury

Antibody Antigen Host Company Catalog # Dilution
Primary antibody (clone #)
   gp91phox Human gp91 Rabbit See ref 18   4,000 (IB)
200 (IHC)
   p22phox Human p22 Rabbit See ref 18   3,000
   iNOS Mouse iNOS Rabbit Transduction Laboratories (Lexington, KY) N32030 10,000
   Ym1 Mouse Ym1 Rabbit StemCell Tech (Vancouver, BC, Canada) 01404 1,000
   GAPDH (6C5) Rabbit GAPDH Mouse Chemicon International (Temecula, CA) MAB374 3,000
   β-Actin (AC-74) Mouse β-Actin Mouse Sigma (St Louise, MO) A5316 4,000
   CD11b (5C6) Mouse CD11b Rat Serotec (Oxford, UK) MCA711 500
   GFAP (G-A-5) Mouse GFAP Mouse Sigma (St Louise, MO) G3893 1000
   NeuN Mouse NeuN Mouse Chemicon International (Temecula, CA) MAB377 1000
   3-NT Nitrated KLH Rabbit Upstate Biotechnology (Lake Placid, NY) 06-284 100
Secondary antibody (conjugation)
   Mouse IgG (HRP) Mouse IgG Sheep GE Healthcare Bioscience (Little Chalfont, UK) NA931 2,000
   Rabbit IgG (HRP) Rabbit IgG Donkey GE Healthcare Bioscience (Little Chalfont, UK) NA934 3,000
   Rabbit IgG (biotinylated) Rabbit IgG Goat Santa Cruz Biotechnology (Santa Cruz, CA) SC-2040 200
   Mouse IgG (Alexa 546) Mouse IgG Goat Molecular Probes (Eugene, OR) A11030 400
   Rabbit IgG (Alexa 488 or 546) Rabbit IgG Goat Molecular Probes (Eugene, OR) A11034 or 11035 400
   Rat IgG (Alexa 546) Rat IgG Goat Molecular Probes (Eugene, OR) A11081 400