Table 1 Antibodies used for immunoblotting (IB) and immunohistochemistry (IHC)
From: Gp91phox (NOX2) in classically activated microglia exacerbates traumatic brain injury
Antibody | Antigen | Host | Company | Catalog # | Dilution |
|---|---|---|---|---|---|
Primary antibody (clone #) | |||||
   gp91phox | Human gp91 | Rabbit | See ref 18 |  | 4,000 (IB) 200 (IHC) |
   p22phox | Human p22 | Rabbit | See ref 18 |  | 3,000 |
   iNOS | Mouse iNOS | Rabbit | Transduction Laboratories (Lexington, KY) | N32030 | 10,000 |
   Ym1 | Mouse Ym1 | Rabbit | StemCell Tech (Vancouver, BC, Canada) | 01404 | 1,000 |
   GAPDH (6C5) | Rabbit GAPDH | Mouse | Chemicon International (Temecula, CA) | MAB374 | 3,000 |
   β-Actin (AC-74) | Mouse β-Actin | Mouse | Sigma (St Louise, MO) | A5316 | 4,000 |
   CD11b (5C6) | Mouse CD11b | Rat | Serotec (Oxford, UK) | MCA711 | 500 |
   GFAP (G-A-5) | Mouse GFAP | Mouse | Sigma (St Louise, MO) | G3893 | 1000 |
   NeuN | Mouse NeuN | Mouse | Chemicon International (Temecula, CA) | MAB377 | 1000 |
   3-NT | Nitrated KLH | Rabbit | Upstate Biotechnology (Lake Placid, NY) | 06-284 | 100 |
Secondary antibody (conjugation) | |||||
   Mouse IgG (HRP) | Mouse IgG | Sheep | GE Healthcare Bioscience (Little Chalfont, UK) | NA931 | 2,000 |
   Rabbit IgG (HRP) | Rabbit IgG | Donkey | GE Healthcare Bioscience (Little Chalfont, UK) | NA934 | 3,000 |
   Rabbit IgG (biotinylated) | Rabbit IgG | Goat | Santa Cruz Biotechnology (Santa Cruz, CA) | SC-2040 | 200 |
   Mouse IgG (Alexa 546) | Mouse IgG | Goat | Molecular Probes (Eugene, OR) | A11030 | 400 |
   Rabbit IgG (Alexa 488 or 546) | Rabbit IgG | Goat | Molecular Probes (Eugene, OR) | A11034 or 11035 | 400 |
   Rat IgG (Alexa 546) | Rat IgG | Goat | Molecular Probes (Eugene, OR) | A11081 | 400 |