α is increased by ischemia in cPLA
α+/+ neurons. A: Immunofluorescence of cPLA2α (green) and fluorescent Nissl dye (blue) in brain slices from cPLA2α+/+ and cPLA2α-/- mice after sham surgery, 2-hour MCAO, or 2-hour MCAO and 2-hour reperfusion. Images from the ischemic core and penumbra regions from the ipsilateral and contralateral hemispheres of each genotype are shown. B: Immunofluorescence of Ser505-phosphorylated cPLA2α (green) in cPLA2α+/+ mice subjected to sham surgery (a-d), 2 hours of MCAO (e-h) or 2 hours of MCAO and 2 hours of reperfusion (i-l). Fluorescent Nissl staining of neurons is in blue. Representative images, n = 3 mice of each genotype. Ipsi, ipsilateral; Contra, contralateral.