α is increased in the ischemic hemisphere following focal ischemia. A: A representative Western blot of cPLA2α+/+ brain protein fractions (25 μg each lane) from the non-ischemic (contralateral) and ischemic (ipsilateral) hemispheres. The same membrane was exposed to antibody and developed sequentially for phospho-cPLA2α, cPLA2α, and actin. The panel on the left represents positive controls-recombinant human cPLA2α (rh cPLA2α) and mouse spleen protein-and a negative control, cPLA2α-/- brain protein. The antibody directed against cPLA2α recognizes both the phosphorylated and nonphosphorylated cPLA2α. B, C: Densitometry analysis of Western blots for (B) total cPLA2α (n = 5 experiments) and (C) phospho-cPLA2α (n = 5 experiments). * P < 0.05 ipsilateral compared to contralateral.