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Table 2 Oxidative stress in cPLA2α+/+ and -/- cortex after MCAO, MCAO and reperfusion, or sham surgery

From: Cytosolic phospholipase A2alpha amplifies early cyclooxygenase-2 expression, oxidative stress and MAP kinase phosphorylation after cerebral ischemia in mice

  cPLA2α+/+ HE area (relative units) cPLA2α-/- HE area (relative units)
  Ipsilateral Contralateral Ipsilateral Contralateral
  Core Penumbra Core Penumbra Core Penumbra Core Penumbra
0 h Reperfuse 45.02 ± 9.58† 38.36 ± 8.53† 6.99 ± 3.88 8.07 ± 4.70 12.76 ± 6.83 12.06 ± 5.85 4.66 ± 4.17 6.27 ± 3.83
2 h Reperfuse 33.01 ± 8.59† 30.70 ± 5.14† 8.82 ± 5.96 9.62 ± 5.95 20..09 ± 5.50 19.47 ± 6.72 6.60 ± 2.98 6.80 ± 4.09
Sham 6.32 ± 5.46 6.03 ± 5.03 5.63 ± 3.71 6.40 ± 4.65 4.11 ± 4.48 4.88 ± 5.43 3.85 ± 3.54 5.05 ± 5.67
  1. Oxidative stress was measured in the cortex of cPLA2α+/+ and -/- brains using micro-fluorescence and threshold densitometry. At the beginning of MCAO or sham surgery 10 mg/kg dihydroethedium (HE) was injected into the relative jugular vein of each mouse. The intensity of HE staining in each region is normalized for area and expressed in relative units. n = 3 mice per condition with analysis of high resolution confocal z-plane images from each mouse as described in the text. †, P < 0.01 cPLA2α+/+ compared to -/- at the same condition.