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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Central Administration of Lipopolysaccharide Induces Depressive-like Behavior in Vivo and Activates Brain Indoleamine 2,3 Dioxygenase In Murine Organotypic Hippocampal Slice Cultures

Figure 3

Proinflammatory cytokines and iNOS expression in hippocampal slices at the mRNA and protein level. Day 0 represents freshly-isolated hippocampi that were subjected to transversal slicing and mRNA being prepared immediately following slicing. Culture media and slice tissue were collected on days 1, 3, 7, 10 and 14 after the start of the culture. LPS (10 ng/ml) was used as a positive control on day 10, with mRNA and protein being measured 6 h later. Steady-state expression of mRNA transcripts was measured by real-time RT-PCR, and proinflammatory cytokines in the media were measured by ELISA. Average Ct values for at 1, 3, 7, 10 and 14 days for each mRNA species were, respectively: TNFα: 27.6 ± 0.7, 27.8 ± 0.5, 29.0 ± 0.3, 29.1 ± 0.5, 28.7 ± 0.6; IL-6: 30.7 ± 0.4, 34.8 ± 0.2, 36.1 ± 0.9, 35.8 ± 0.8, 36.6 ± 1.1; iNOS: 28.8 ± 0.8, 31.8 ± 0.8, 31.1 ± 1.0, 32.9 ± 0.3, 35.5 ± 0.8. Data represent the mean ± SEM (n = 3 in each group). Bars labeled with different letters (a, b, c or d) are significantly different from each other at p < 0.05.

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Journal of Neuroinflammation

ISSN: 1742-2094