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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Central Administration of Lipopolysaccharide Induces Depressive-like Behavior in Vivo and Activates Brain Indoleamine 2,3 Dioxygenase In Murine Organotypic Hippocampal Slice Cultures

Figure 5

LPS induces proinflammatory cytokines and iNOS expression in OHSCs in a dose- and time-dependent manner. (A) 1, 10 or 100 ng/ml LPS were added to the medium after 10 days in culture. Tissue and media were collected 6 h later. Average Ct values for 1, 10 or 100 ng/ml LPS were, respectively, for TNFα: 20.6 ± 0.6, 19.6 ± 0.5, 19.6 ± 0.6; IL-6: 24.1 ± 0.6, 22.5 ± 0.6, 22.1 ± 0.6; iNOS: 26.2 ± 0.9, 24.9 ± 1.0, 23.6 ± 0.9. (B) Hippocampal slices were treated with LPS (10 ng/ml) and tissue and media were collected at various times. Average Ct values at 6, 12, 18 and 24 h were, respectively, for TNFα: 19.7 ± 0.1, 22.8 ± 0.7, 24.4 ± 0.9, 25.3 ± 0.7; IL-6: 23.2 ± 0.3, 24.9 ± 0.7, 28.1 ± 1.1, 28.8 ± 0.7; iNOS: 24.1 ± 0.1, 22.7 ± 0.6, 24.4 ± 1.1, 25.2 ± 1.0. Amount of the proinflammatory cytokines was measured by ELISA. Bars represent the mean ± SEM (n = 3 in each group). Bars labeled with different letters (a, b, c, d or e) are significantly different from each other at p < 0.05.

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