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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Central Administration of Lipopolysaccharide Induces Depressive-like Behavior in Vivo and Activates Brain Indoleamine 2,3 Dioxygenase In Murine Organotypic Hippocampal Slice Cultures

Figure 6

LPS increases IDO mRNA and enzymatic activity in OHSCs. (A) 1, 10 or 100 ng/ml LPS were added to the medium after 10 days in culture. Tissue and media were collected 6 h following addition of LPS. Average Ct values for 1, 10 or 100 ng/ml LPS for IDO were, respectively, 38.4 ± 0.8, 36.4 ± 0.3, 36.4 ± 0.3. (B) Hippocampal slices were treated with LPS (10 ng/ml) for various times. Average Ct values at 6, 12, 18 and 24 h for IDO were, respectively, 36.7 ± 0.4, 39.5 ± 0.5, 39.7 ± 0.4, 40 ± 0. (C) Hippocampal slices were treated with LPS (10 ng/ml) for 8, 12, 18 and 24 h. IDO enzymatic activity was determined by measuring the amount of kynurenine formed upon incubation of slices lysates with exogenous L-tryptophan. IDO enzymatic activity was expressed as a percentage of the medium control. Bars labeled with different letters (a or b) are significantly different from each other at p < 0.05.

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Journal of Neuroinflammation

ISSN: 1742-2094