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Table 1 Rotenone and 3-NP do not affect cell viability

From: Effects of mitochondrial dysfunction on the immunological properties of microglia

  WST-1 (% of basal)
Condition Control LPS IL-4 LPS + IL-4
Control 100 174 ± 24 121 ± 19 156 ± 24
0.3 mM 3-NP 96 ± 24 158 ± 23 89 ± 24 132 ± 29
1 mM 3-NP 76 ± 25 141 ± 42 107 ± 31 115 ± 29
0.02 μM Rotenone 146 ± 12 194 ± 42 118 ± 21 132 ± 26
0.2 μM Rotenone 115 ± 21 115 ± 38 114 ± 33 111 ± 27
  1. Microglial cells were treated with rotenone or 3-NP and stimulated with 10 ng/ml IL-4. After 24 hrs, 1 μg/ml LPS was added for 18 hrs. The WST-1 assay was performed as described in Methods. Values are mean ± SEM for independent toxicity measurements. n = 6 wells (i.e., 3 separate experiments performed in duplicate); not significantly different from control (ANOVA followed by Dunnett's Multiple Comparison test).