Figure 4

Resveratrol inhibits LPS-induced cytokine mRNA expression in glial cells. N9 cells (A), murine primary microglial cells (B), or astrocytes (C) were incubated with 0.5 μg/ml LPS in the presence or absence of different concentrations of resveratrol (RV) for 8 h. Cells were collected, and RNA was extracted to detect TNF-α, IL-1β, IL-6 and MCP-1 mRNA by RT-PCR. Data are presented as mean ± SD for three independent experiments. *P < 0.05, **P < 0.01 compared with treatment with LPS alone. Representative gels are shown.