Cell-surface expression of specific (CD66b; A) and azurophilic (CD63; B) granular proteins on fresh polymorphonuclear neutrophils (PMNs), assessed by whole blood immunofluorescence flow cytometry. Results were expressed as mean linear fluorescence intensity (MFI ± SEM; bars) in arbitrary units (a.u.) and the percentage of receptor-positive cells (circles). Blood was sampled serially from severe TBI patients resuscitated with normal saline (NS, n = 13) vs hypertonic saline-dextran (HSD, n = 10), upon hospital admission (Adm) and 12, 24, and 48-h post-resuscitation, and from healthy controls (n = 25). *P < 0.05 vs age-matched healthy controls (n = 25); †P < 0.05 vs time-matched NS-treated patients by ANOVA.