Figure 1

Interactions of astrocytes and neutrophils. A. Immunocytochemical staining of F4/80 (panel 1 in red) and GFAP (panel 2 in green) in primary astrocyte culture. Cells were counterstained with DAPI (panel 3 in blue). The merged image is shown in panel 4. B. Differential interference contrast images of neutrophils cultured alone or co-cultured with astrocytes for 1.5 hr. 1. Resting neutrophils alone; 2. LPS-activated neutrophils; 3. Resting neutrophils co-cultured with resting astrocytes; 4. Resting neutrophils co-cultured with poly I:C-primed astrocytes. Arrows indicate astrocytes.