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Figure 2 | Journal of Neuroinflammation

Figure 2

From: The role of the JAK2-STAT3 pathway in pro-inflammatory responses of EMF-stimulated N9 microglial cells

Figure 2

Localization of CD11b and p-STAT3 immunoreactivity in activated N9 cells. Experiments were performed as described above. Confocal immunofluorescence microscopy was performed on cultures that were immunoreacted with antibodies against phospho-STAT3 tyr705 (left, rabbit monoclonal, 1:100; FITC-conjugated sheep anti- rabbit secondary, 1:200) and CD11b (right, as Figure 1 shows) at 12 h after EMF exposure (A-L). (A-C) Untreated cultures were used as a control. (D-F) Cultures pretreated with P6 (10 μM). (G-I) EMF induced more phosphorylation of STAT3 (green) and expression of CD11b (red) in N9 cells. (J-L) P6 inhibits the phosphorylation of STAT3 and expression of CD11b at 12 h after EMF exposure. (M-O) P6 pretreatment significantly suppresses the phosphorylation of STAT3, but a slight and significant increase of CD11b is still apparent at 3 h after EMF exposure. Scale bar 10 μm. (P) Bar graphs show semi-quantification of fluorescence intensity for p-STAT3 and CD11b in N9 cells. Results are presented as mean ± S.D. of three independent experiments. Statistical comparisons to control are indicated by * p < 0.05; ** p < 0.01.

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