Figure 2

ND02 inhibits TNF-α-induced phosphorylation of NF-κB and IκB-α through an Akt signaling pathway. SH-SY5Y cells were pre-incubated with PI3 kinase inhibitor, ly294002 (30 μM) or ND02 (20 μ M or 40 μ M) for 30 min and then incubated with TNF-α (20 ng/ml) for 15 min. Phospho-Akt, Phospho-IκB-α and Phospho-NF-κB expression were analyzed by western blotting. Results are expressed as fold increase over control, n = 5. ^aP < 0.05, ^bP < 0.01 vs. TNF-α-induced group. ^cP < 0.01 vs. control group. Significance was determined by one-way analysis of ANOVA followed by Dunnett's test.