Figure 3

Exaggerated YM1 Activation in rTg4510 mice. Immunohistochemical staining for the microglial alternative activation marker YM1 in cortex, hippocampus (CA1), and entorhinal cortex (ECX) was performed. rTg4510 mice or nontransgenic (nTg) littermates were injected with LPS or vehicle into the anterior cortex and hippocampus. Images (A-D) were collected from the anterior cortex of nontransgenic (A, C) or rTg4510 mice (B, D) injected with either vehicle (A, B) or LPS (C, D), from stratum radiatum of CA1 of nontransgenic (F, H) or rTg4510 mice (G, I) after vehicle (F, G) or LPS (H, I) injections, or from entorhinal cortex (ECX) of nontransgenic (K, M) or rTg4510 mice (L, N) which received vehicle (K,L) or LPS (M, N) injections one week previously. Mean ± S.E.M (n = 6-8) of % Area for immunostaining of YM1+ microglia (black) in the anterior cortex, CA1, and ECX are presented in E, J, and O. YM1+ staining increased in CX, CA1, and ECX of mice treated with LPS compared to vehicle-treated mice. Inductions in rTg4510 mice were larger than in nontransgenic littermates. Statistical analysis was performed using 2-way ANOVA followed by Fisher's PLSD multiple comparison test (*p < 0.05), n = 6-8. Scale bar represents 20 μm.