Figure 6

A second phospho-tau epitope was also increased after LPS. Immunohistochemical staining of tau phosphorylated at Ser396 in cortex anterior cortex (A-D), hippocampus (CA1; F-I), and entorhinal cortex (ECX; K-N) after injections of either vehicle (A, C, F, H, K, M) or LPS (B, D, G, I, L, N) in nontransgenic (A, C, F, H, K, M) or rTg4510 (B, D, G, I, L, N) mice. The % Area for immunostaining of phospho-tau Ser396 (mean ± S.E.M; n = 6-8) is presented for each brain region in E, J, and O. Phospho-tau Ser396 staining increased in anterior cortex, CA1, and ECX of rTg4510 mice treated with LPS compared to vehicle-treated mice. No staining was detected in nontransgenic littermates. Statistical analysis was performed using 2-way ANOVA followed by Fisher's PLSD multiple comparison test (*p < 0.05), n = 6-8. Scale bar represents 20 μm.