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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Phagocytosis and LPS alter the maturation state of β-amyloid precursor protein and induce different Aβ peptide release signatures in human mononuclear phagocytes

Figure 1

Release of Aβ x-40 by mononuclear phagocytes is induced by phagocytosis of polystyrene beads or acetylated LDL. Human mononuclear phagocytes were freshly isolated by density gradient centrifugation followed by depletion of all non phagocytes with magnetic activated cell sorting. Cells were resuspended in AIM-V medium and seeded on ultra-low attachment plates. They were left either untreated (CON) or stimulated with 4*107/ml polystyrene beads (PB) or 10 μg/ml acLDL (acLDL). A βx-40 was assessed in supernatants after 72 h in vitro with a sandwich ELISA using the mab BNT77 (binding AA11-28 of Aβ) as the capturing antibody and horseradish peroxidase conjugated F(ab') fragments of the BA27 (specific for the C-terminus of Aβ40) as the detection antibody. Note the 8-11 fold increased release of Aβx-40 after treatment of mononuclear phagocytes with polystyrene beads or acLDL. (p < 0,01, one-way ANOVA followed by Dunnett's multiple comparison test)

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