Figure 6
Total RNA extracted from brain microvessels of 6, 12, 18 and 24 month old rats was reverse transcribed and amplified with (a) IL-1β or (b) IL-6 specific primers. Amplified products were visualized in 1.5% agarose gel and band intensity represented as bars. Data were normalized relative to GAPDH expression. Data are mean ± SD from 3 experiments performed in triplicate. *p < 0.05 vs. 6 months; **p < 0.01 vs. 6 months.