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Table 1 Parameters of the stereological analysis

From: Genetic background modifies neurodegeneration and neuroinflammation driven by misfolded human tau protein in rat model of tauopathy: implication for immunomodulatory approach to Alzheimer's disease

   

dissector

sampling

      
   

base

height

Grid

UGZ

BA

t

   

Structure quantified (antibody)

OBJ

ssf

(μm × μm)

(μm)

(μm × μm)

(μm)

(μm)

(μm)

∑OD

∑Q-

CE

Neurofibrillar tangles (AT8)

60 ×

1/13

100 × 100

15

370 × 370

1.5

40

21.85

3296.70

394.80

0.060

Microglia (Iba1)

60 ×

1/13

70 × 70

15

860 × 860

1.5

40

24.41

767.20

1115.40

0.030

Macrophage-like structures (Iba1)

60 ×

1/13

70 × 70

15

860 × 860

1.5

40

24.41

767.20

96.00

0.260

MHCII-positive microglia - frequent (MHCII)

60 ×

1/13

100 × 100

15

500 × 500

1.5

40

25.60

2645.40

1861.40

0.041

MHCII-positive microglia - sparse (MHCII)

60 ×

1/13

100 × 100

15

370 × 370

1.5

40

26.54

4029.20

252.20

0.085

Astrocytes (GFAP)

60 ×

1/13

70 × 70

15

860 × 860

1.5

40

24.66

680.19

1696.05

0.024

Neurons (NeuN)

60 ×

1/13

70 × 70

15

650 × 650

1.5

40

20.70

1243.76

1655.81

0.047

  1. Abbreviations: OBJ - objective; ssf - section sampling fraction; UGZ - upper guard zone; BA - block advance (section thickness when cut); t - average number-weighted section thickness after immunohistochemical processing; ∑OD - average sum of optical dissector used; ∑Q- - average number of markers counted; CE - coefficient of error (Schmitz-Hof).