Figure 2
From: CCL2 modulates cytokine production in cultured mouse astrocytes
IL-6 mRNA expression is exacerbated in Ccl2 -/- astrocytes following LPS stimulation. Expression of IL-6 mRNA was measured by qPCR in cell lysates following stimulation with 1 μg/ml LPS for 8 h. Whilst mRNA levels were minimal in unstimulated cells, IL-6 expression was strongly induced by LPS exposure in astrocytes from both strains (# p < 0.001; 2-way ANOVA effect of treatment). Furthermore, significantly higher expression of IL-6 mRNA was detected in Ccl2-/- astrocytes after LPS treatment compared to LPS-treated wild-type cells (* p < 0.05). Data represent mean + sem of three separate experiments assayed in triplicate.